Journal: Frontiers in immunology

Article Title: Evaluation of Immune and Vaccine Competence in Steroid-Sensitive Nephrotic Syndrome Pediatric Patients.

doi: 10.3389/fimmu.2021.602826

Figure Lengend Snippet: FIGURE 2 | Total and antigen-specific IgG-secreting B cells in steroid-sensitive nephrotic syndrome pediatric patients at onset. (A–E) Isolated PBMCs were stimulated for 5 days with CpG plus rhIL-21 and rhIL-4. Following stimulation, (A) total, (B,C) anti-tetanus and (D,E) anti-HBV IgG-secreting B cells were enumerated by ELISPOT in steroid-sensitive nephrotic syndrome pediatric patients at disease onset (SSNS, n=11) and in age-matched controls (CTRL, n=5). Antigen-specific memory B cells were represented as (B,D) absolute count/106 cells and as (C,E) percentage of total IgG-secreting B cells. Each plot represents a different patient. Horizontal lines indicate the means and differences between groups were compared using the unpaired t test.

Article Snippet: For the simultaneous detection of IgM and IgG we used the Human IgG/IgM Dual-Color B Cell ELISpot Kit (R&D System).

Techniques: Isolation, Enzyme-linked Immunospot